Immunohistochemistry:Article Title: Dysfunctional EGFR and oxidative stress-induced PKD1 signaling drive formation of DCLK1+ pancreatic stem cells
Article Snippet: Antibody Company/Source Catalog Number Species IHC IF WB FACS IHC-IF Zenon 4-HNE Alpha Diagnostic International HNE12-S goat 1:300 acetylated α-tubulin Sigma T6793 mouse 1:2000 1:2000, 1:5000 CD133 Proteintech 18470-1-AP rabbit 1:1000 cleaved Caspase-3 Cell Signaling Technologies 9661S rabbit 1:100 DCLK1 Abcam ab37994 rabbit 1:250, 1:2000 1:250, 1:1000 1:50 1:340 DCLK1 R&D Systems AF7138 sheep 1:250 1:250 LAMP-1 Abcam ab24170 rabbit 1:2000 OCT4 Abcam ab18976 rabbit 1:100 PKD1 Abnova H090005587-A01 mouse 1:200 PKD1 Everest Biotech EB12885 goat 1:500 1:500 1:200 POU2F3 LifeSpan BioSciences LS Bio C336168 rabbit 1:50 pT202/pY204-ERK1/2 Cell Signaling Technologies 4370S rabbit 1:800 1:200 pY1068-EGFR Abcam ab40815 rabbit 1:1000 pY95-PKD Storz Laboratory rabbit 1:100 β-endorphin Immunostar 20063 rabbit 1:2000 1:2000 Supplementary Table S1 Supplementary Table S1, Related to Figures 1-7 and Supplemental Figures S1-S5: Antibodies and dilutions used.
Western Blot:Article Title: Dysfunctional EGFR and oxidative stress-induced PKD1 signaling drive formation of DCLK1+ pancreatic stem cells
Article Snippet: Antibody Company/Source Catalog Number Species IHC IF WB FACS IHC-IF Zenon 4-HNE Alpha Diagnostic International HNE12-S goat 1:300 acetylated α-tubulin Sigma T6793 mouse 1:2000 1:2000, 1:5000 CD133 Proteintech 18470-1-AP rabbit 1:1000 cleaved Caspase-3 Cell Signaling Technologies 9661S rabbit 1:100 DCLK1 Abcam ab37994 rabbit 1:250, 1:2000 1:250, 1:1000 1:50 1:340 DCLK1 R&D Systems AF7138 sheep 1:250 1:250 LAMP-1 Abcam ab24170 rabbit 1:2000 OCT4 Abcam ab18976 rabbit 1:100 PKD1 Abnova H090005587-A01 mouse 1:200 PKD1 Everest Biotech EB12885 goat 1:500 1:500 1:200 POU2F3 LifeSpan BioSciences LS Bio C336168 rabbit 1:50 pT202/pY204-ERK1/2 Cell Signaling Technologies 4370S rabbit 1:800 1:200 pY1068-EGFR Abcam ab40815 rabbit 1:1000 pY95-PKD Storz Laboratory rabbit 1:100 β-endorphin Immunostar 20063 rabbit 1:2000 1:2000 Supplementary Table S1 Supplementary Table S1, Related to Figures 1-7 and Supplemental Figures S1-S5: Antibodies and dilutions used.
FACS:Article Title: Dysfunctional EGFR and oxidative stress-induced PKD1 signaling drive formation of DCLK1+ pancreatic stem cells
Article Snippet: Antibody Company/Source Catalog Number Species IHC IF WB FACS IHC-IF Zenon 4-HNE Alpha Diagnostic International HNE12-S goat 1:300 acetylated α-tubulin Sigma T6793 mouse 1:2000 1:2000, 1:5000 CD133 Proteintech 18470-1-AP rabbit 1:1000 cleaved Caspase-3 Cell Signaling Technologies 9661S rabbit 1:100 DCLK1 Abcam ab37994 rabbit 1:250, 1:2000 1:250, 1:1000 1:50 1:340 DCLK1 R&D Systems AF7138 sheep 1:250 1:250 LAMP-1 Abcam ab24170 rabbit 1:2000 OCT4 Abcam ab18976 rabbit 1:100 PKD1 Abnova H090005587-A01 mouse 1:200 PKD1 Everest Biotech EB12885 goat 1:500 1:500 1:200 POU2F3 LifeSpan BioSciences LS Bio C336168 rabbit 1:50 pT202/pY204-ERK1/2 Cell Signaling Technologies 4370S rabbit 1:800 1:200 pY1068-EGFR Abcam ab40815 rabbit 1:1000 pY95-PKD Storz Laboratory rabbit 1:100 β-endorphin Immunostar 20063 rabbit 1:2000 1:2000 Supplementary Table S1 Supplementary Table S1, Related to Figures 1-7 and Supplemental Figures S1-S5: Antibodies and dilutions used.
Immunohistochemistry-IF:Article Title: Dysfunctional EGFR and oxidative stress-induced PKD1 signaling drive formation of DCLK1+ pancreatic stem cells
Article Snippet: Antibody Company/Source Catalog Number Species IHC IF WB FACS IHC-IF Zenon 4-HNE Alpha Diagnostic International HNE12-S goat 1:300 acetylated α-tubulin Sigma T6793 mouse 1:2000 1:2000, 1:5000 CD133 Proteintech 18470-1-AP rabbit 1:1000 cleaved Caspase-3 Cell Signaling Technologies 9661S rabbit 1:100 DCLK1 Abcam ab37994 rabbit 1:250, 1:2000 1:250, 1:1000 1:50 1:340 DCLK1 R&D Systems AF7138 sheep 1:250 1:250 LAMP-1 Abcam ab24170 rabbit 1:2000 OCT4 Abcam ab18976 rabbit 1:100 PKD1 Abnova H090005587-A01 mouse 1:200 PKD1 Everest Biotech EB12885 goat 1:500 1:500 1:200 POU2F3 LifeSpan BioSciences LS Bio C336168 rabbit 1:50 pT202/pY204-ERK1/2 Cell Signaling Technologies 4370S rabbit 1:800 1:200 pY1068-EGFR Abcam ab40815 rabbit 1:1000 pY95-PKD Storz Laboratory rabbit 1:100 β-endorphin Immunostar 20063 rabbit 1:2000 1:2000 Supplementary Table S1 Supplementary Table S1, Related to Figures 1-7 and Supplemental Figures S1-S5: Antibodies and dilutions used.
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